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Apparatus and method for measuring the fluorescenc

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专利名称:Apparatus and method for measuring the

fluorescence of large multi-cellularorganisms

发明人:Paul E. Johnson申请号:US11985038申请日:20071113

公开号:US20080129998A1公开日:20080605

专利附图:

摘要:Apparatus and methods for measuring the fluorescence of large multi-cellularorganisms in a sample of liquid includes a pumping mechanism, a fluorescence measuring

device, a method of analyzing the measurements, and optionally, a sorting mechanism.The pumping mechanism transfers large multi-cellular organisms from a reservoirthrough a fluorescence-measuring device causing minimum physical damage and/orstress. The pressure differential driving the organisms from a sample container/reservoirthrough the measuring device can be derived from gravity, air pressure, or liquidpressure, or some combination of the three. The fluorescence can be measured in acytometer using a light detector or imager. Generally the detection element will includea filter, isolating the wavelength of fluorescent emission. The illumination may beprovided by a laser or by an LED, combined with the use of dichroic mirrors to allowmultiple wavelength simultaneous illumination. In addition, an LED will typically be usedwith a filter to isolate the optimum excitation wavelength while blocking

scattered/reflected LED light from the detection element (but not the fluorescence).

申请人:Paul E. Johnson

地址:Laramie WY US

国籍:US

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